HemaTrack™ is a UMI-based multiplexed TCR/BCR profiling assay for MRD quantification in ALL. QuaSIR™, the analysis workflow arm of HemaTrack™ analyzes TCR/BCR NGS data and generates comprehensive reports to aid in the diagnosis and treatment of ALL. The assay demonstrates linearity in dilution experiments using leukemic cell lines for TCR/BCR profiling. The combined HemaTrack™ and QuaSIR™ workflow achieves excellent sensitivity and specificity when benchmarked against RQ-PCR in retrospective analysis of bio-banked ALL samples. This integrated workflow enables broader application of NGS-based MRD detection in clinical settings. The utility and accuracy of HemaTrack™ will be evaluated in an upcoming multicenter prospective clinical trial.